Journal:

Article Title: Enhancement of lectin pathway haemolysis by immunoglobulins

doi: 10.1046/j.1365-2249.1999.00996.x

Figure Lengend Snippet: Enhancement of lectin pathway-mediated haemolysis by cosensitization with purified human IgM, IgG or IgA prior to lysis in human serum-Mg-EDTA. Each isotype resulted in significant enhancement of MBL-initiated haemolysis. E-M-MBL were cosensitized with human IgM, IgG or IgA using chromic chloride prior to incubation in serum-Mg-ethyleneglycol tetraacetic acid. A maximum 2.5-fold enhancement of haemolysis (from 9 to 26 LH50 U/ml) was observed with IgM and IgA cosensitization, while IgG led to approx. fourfold enhancement, from 9 to 40 LH50 U/ml.

Article Snippet: Purified rabbit anti-sheep erythrocyte IgM and IgG were purchased from Accurate Chemical and Scientific Corporation (Westbury, NY, USA).

Techniques: Purification, Lysis, Incubation

Journal:

Article Title: Enhancement of lectin pathway haemolysis by immunoglobulins

doi: 10.1046/j.1365-2249.1999.00996.x

Figure Lengend Snippet: EA-M-MBL were prepared by incubation (15 min, 37°C) of E-M-MBL with IgM and IgG haemolysin, respectively, in Mg-ethyleneglycol tetraacetic acid (EGTA), washed in Mg-EGTA, and tested for lectin pathway haemolysis in human serum-Mg-EGTA. A twofold enhancement (from 9 to 20 LH50 U/ml) was observed upon presensitization with IgM, while fourfold increase (from 9 to 40 LH50 U/ml) was observed upon presensitization with IgG.

Article Snippet: Purified rabbit anti-sheep erythrocyte IgM and IgG were purchased from Accurate Chemical and Scientific Corporation (Westbury, NY, USA).

Techniques: Incubation

Journal: Journal of biomedical materials research. Part B, Applied biomaterials

Article Title: In vivo assessment of two endothelialization approaches on bioprosthetic valves for the treatment of chronic deep venous insufficiency

doi: 10.1002/jbm.b.33507

Figure Lengend Snippet: (a) SIS punches modified with biotin and streptavidin were exposed to I125 radiolabeled biotinylated KDR. Radioactivity of the punches was measured to give a quantification of bound biotinylated anti-KDR. (b) Modified SIS punches were incubated with increasing concentrations of biotinylated anti-KDR and then exposed to I125 radiolabeled recombinant human KDR. Radioactivity of the punches was measured to give a quantification of bound KDR protein. All data is presented as the mean ± standard deviation, n=4.

Article Snippet: Antibody orientation on SIS Vacuum pressed SIS sheets were provided by Cook Biotech, Inc. SIS was biotinylated by treating with a 0.5 mg/mL solution of EZ-LinkTM Sulfo NHS-LC-biotin (Thermo Scientific) in PBS for 24 hrs at room temperature.

Techniques: Modification, Radioactivity, Incubation, Recombinant, Standard Deviation